The mechanism's assistance contributed to the restoration of the fins, in contrast to the standard mechanism of tromorphosis.
Scientists from the University Delegates (USA) conducted experiments with frogs showed that using a bioreactor that provides progesterone, it is possible to restore the similarity to the organ in a close source manner.
For the experiments, the researchers chose a smooth African frog (Xenopus laevis). With the loss of this organ can grow replacement, not having the same characteristics. The usual example looks like a thorny structure with no fins at the end. The scientists divided them into three groups. The first frog received the bioreactor and transferred the drug to the body. The second group was used for control and the third is not used.
Bioreactor was printed on a 3D printer. It was filled with gidratirovannykh silk proteins promote regeneration. In the first group, the hydrogel is taken to progesterone at the wound site. Progesterone – a steroid hormone that plays a key role during pregnancy and embryonic development in humans and other animals. In the third group bioreactor he is absent. The device is activated for 24 hours immediately after wound formation.
So the biologists watched the frogs for the next nine and a half months, following the growth of new organs. In contrast to the other two groups, frogs showed for the first time greater potential for recovery. Instead of severed legs appeared veslopolova structure that resembled a normal leg, which they enjoyed as well before the habit. To clarify the details of the procedure, the researchers performed molecular and histological analyzes. Bone, blood vessels, and neural tissue were more developed in the experimental group.
Analysis of the transcriptome showed that the bioreactor altered the expression of genes in cells. The processes that led to scarring and immune response were replicated in favor of genes responsible for oxidative stress, serotonergic transmission, and leukocyte activity. In the future, the authors hope to repeat the results of this experiment on mammals.
Previously, scientists at the University of Golfscope have demonstrated how stem cells allow mammals to grow tail along with a lost part of the spinal cord.
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